Stability enhancement of cytochrome c through heme deprotonation and mutations
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چکیده
منابع مشابه
Heme A of Cytochrome c Oxidase
Heme A, isolated from bovine heart muscle by procedures which include extractions into pyridine/ chloroform and two-phase, liquid-liquid chromatography on Celite, has been converted to several derivatives. Examination of the proton nuclear magnetic resonance (PMR) spectra and other properties of these derivatives reveals heme A to be the iron complex of %formyl-6,7-bis(2”-hydroxycarbonylethyl)-...
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A highly efficient in vitro system was established for measuring by high performance liquid chromatography the formation of holocytochrome c by yeast mitochondria. Holocytochrome c formation required reducing agents, of which dithiothreitol was the most effective. With biosynthetically made, pure Drosophila melanogaster apocytochrome c and Saccharomyces cerevisiae mitochondria, the activity of ...
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ژورنال
عنوان ژورنال: Biophysical Chemistry
سال: 2009
ISSN: 0301-4622
DOI: 10.1016/j.bpc.2008.09.020